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Host
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARL Recombinant antibody, PBS Only

PARL Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242171C12

Cat no : 85167-1-PBS

Synonyms

PRO2207, Presenilin-associated rhomboid-like protein, mitochondrial, P-beta, Pbeta, EC:3.4.21.105

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PARL Recombinant antibody (85167-1-RR)

    WB analysis of MCF-7 using 85167-1-RR (same clone as 85167-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85167-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARL Recombinant antibody (85167-1-RR)

    WB analysis using 85167-1-RR (same clone as 85167-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85167-1-RR (PARL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85167-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85167-1-PBS targets PARL in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARL fusion protein Ag24789 相同性解析による交差性が予測される生物種
Full Name presenilin associated, rhomboid-like
Calculated molecular weight 42 kDa
Observed molecular weight45 kDa
GenBank accession numberBC014058
Gene symbol PARL
Gene ID (NCBI) 55486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PARL (Presenilin associated rhomboid like), is a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. PARL has 2 isoforms produced by alternative splicing with the molecular mass of 42 kDa and 37 kDa. PARL regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of PARL may result in the release of a small peptide, P-beta, which may transit to the nucleus. The self-regulated cleavage of PARL at positions 52-53 (α-site) and 77--78 (β-site) produce two cleaved forms of PARL named MAMP and PACT with the molecular mass of 36 kDa and 33 kDa respectively (PMID: 14732705, 28178523).