Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 13371-1-AP
Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13371-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13371-1-AP
Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis using 13371-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13371-1-AP
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 13371-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 13371-1-AP
IP result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.
IHC staining of mouse colon using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13371-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 13371-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 13371-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 13371-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 13371-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of K-562 using 13371-1-AP
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PARP1 (13371-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 13371-1-AP

Publications(676)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (676)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse colon tissue, human breast cancer tissue, human lung cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	HEK-293 cells, MCF-7 cells
Positive FC (Intra) detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 640 publications below
IHC	See 26 publications below
IF	See 18 publications below
IP	See 8 publications below
CoIP	See 4 publications below
ChIP	See 2 publications below

Product Information

13371-1-AP targets PARP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, monkey, chicken, bovine, sheep, fungus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PARP1 fusion protein Ag4193 相同性解析による交差性が予測される生物種
Full Name	poly (ADP-ribose) polymerase 1
Calculated molecular weight	1014 aa, 113 kDa
Observed molecular weight	113-116 kDa, 89 kDa
GenBank accession number	BC037545
Gene Symbol	PARP1
Gene ID (NCBI)	142
RRID	AB_2160459
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09874
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 13371-1-AP	Download protocol
IHC protocol for PARP1 antibody 13371-1-AP	Download protocol
IF protocol for PARP1 antibody 13371-1-AP	Download protocol
IP protocol for PARP1 antibody 13371-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P Authors - Yuan-Li Ai View Article
human	WB	Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. Authors - Jiangfeng Li View Article
human	WB	ACS Nano PMID: 31670945 Authors - Ying Xu View Article
mouse	WB	Exp Mol Med Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury Authors - Zhenjun Yu View Article
human	WB	Nat Commun The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Authors - Guangjian Fan View Article
human	WB	Nat Commun Loss of KLF14 triggers centrosome amplification and tumorigenesis. Authors - Guangjian Fan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13371-1-AP

WB analysis using 13371-1-AP

WB analysis of HeLa using 13371-1-AP

WB analysis using 13371-1-AP

WB analysis of HeLa using 13371-1-AP

WB analysis of C6 using 13371-1-AP

IP experiment of K-562 using 13371-1-AP

IHC staining of mouse colon using 13371-1-AP

IHC staining of mouse testis using 13371-1-AP

IHC staining of human lung cancer using 13371-1-AP

IHC staining of mouse testis using 13371-1-AP

IHC staining of mouse colon using 13371-1-AP

IHC staining of human breast cancer using 13371-1-AP

IHC staining of human breast cancer using 13371-1-AP

IF Staining of mouse testis using 13371-1-AP

IF Staining of mouse testis using 13371-1-AP

IF Staining of MCF-7 using 13371-1-AP

IF Staining of HEK-293 using 13371-1-AP

FC experiment of K-562 using 13371-1-AP

PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

PMID: 31670945

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury

The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.

Loss of KLF14 triggers centrosome amplification and tumorigenesis.