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  • Featured Product
  • KD/KO Validated

PARP1 Monoclonal antibody, PBS Only

PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7D4

Cat no : 66520-1-PBS

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

    Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of HSC-T6 using 66520-1-Ig (same clone as 66520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

    HeLa cells (20 μg) were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis of RAW 264.7 using 66520-1-Ig (same clone as 66520-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARP1 Monoclonal antibody (66520-1-Ig)

    WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using PARP1 Monoclonal antibody (66520-1-Ig)

    IP experiment of K-562 using 66520-1-Ig (same clone as 66520-1-PBS)

    IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of rat colon using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of mouse testis using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of mouse colon using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PARP1 Monoclonal antibody (66520-1-Ig)

    IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PARP1 Monoclonal antibody (66520-1-Ig)

    IF Staining of Neuro-2a using 66520-1-Ig (same clone as 66520-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PARP1 Monoclonal antibody (66520-1-Ig)

    FC experiment of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using PARP1 Monoclonal antibody (66520-1-Ig)

    FC experiment of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66520-1-PBS targets PARP1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PARP1 fusion protein Ag19173 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight 113-116 kDa, 85-89 kDa
GenBank accession numberBC037545
Gene symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.