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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP1 Recombinant antibody (80174-1-RR)

    WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

    Jurkat cells treated with Staurosporin were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARP1 Recombinant antibody (80174-1-RR)

    WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARP1 Recombinant antibody (80174-1-RR)

    WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PARP1 Recombinant antibody (80174-1-RR)

    IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP1 Recombinant antibody (80174-1-RR)

    IHC staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PARP1 Recombinant antibody (80174-1-RR)

    IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PARP1 Recombinant antibody (80174-1-RR)

    IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PARP1 Recombinant antibody (80174-1-RR)

    IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PARP1 Recombinant antibody (80174-1-RR)

    IF Staining of MCF-7 using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PARP1 Recombinant antibody (80174-1-RR)

    IF Staining of HepG2 using 80174-1-RR (same clone as 80174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80174-1-RR

    Affinity and Kinetic Characterization of 80174-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80174-1-RR against Human PARP1 were performed. The affinity constant is 0.639 nM.

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PARP1 Recombinant antibody, PBS Only

PARP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 80174-1-PBS
Clone No.3N19

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

ARTD1, ADPRT1, ADPRT 1, ADPRT, ADP-ribosyltransferase diphtheria toxin-like 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80174-1-PBS targets PARP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARP1 fusion protein Ag4193 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight113-116, 89 kDa
GenBank accession numberBC037545
Gene Symbol PARP1
Gene ID (NCBI) 142
RRIDAB_2918872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.