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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARP10 Polyclonal antibody

PARP10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26072-1-AP

Synonyms

Poly [ADP-ribose] polymerase 10, PARP-10, PARP 10, EC:2.4.2.-, ARTD10

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of Jurkat using 26072-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of HL-60 using 26072-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PARP10 Polyclonal antibody (26072-1-AP)

    WB analysis of MCF-7 using 26072-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26072-1-AP (PARP10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PARP10 Polyclonal antibody (26072-1-AP)

    IP experiment of Jurkat using 26072-1-AP

    IP result of anti-PARP10 (IP:26072-1-AP, 4ug; Detection:26072-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PARP10 Polyclonal antibody (26072-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PARP10 Polyclonal antibody (26072-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26072-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26072-1-AP (PARP10 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PARP10 Polyclonal antibody (26072-1-AP)

    IF Staining of MCF-7 using 26072-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PARP10 antibody (26072-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PARP10 Polyclonal antibody (26072-1-AP)

    IF Staining of HepG2 using 26072-1-AP

    Immunofluorescent analysis of HepG2 cells using 26072-1-AP (PARP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26072-1-AP targets PARP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP10 fusion protein Ag23457 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 10
Observed molecular weight 130-140 kDa
GenBank accession numberBC136542
Gene symbol PARP10
Gene ID (NCBI) 84875
RRIDAB_2880362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PARP10 antibody 26072-1-APDownload protocol
IHC protocol for PARP10 antibody 26072-1-APDownload protocol
IF protocol for PARP10 antibody 26072-1-APDownload protocol
IP protocol for PARP10 antibody 26072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols