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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using PARP14 Polyclonal antibody (30127-1-AP)

    IHC staining of human placenta using 30127-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30127-1-AP (PARP14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PARP14 Polyclonal antibody (30127-1-AP)

    IHC staining of human placenta using 30127-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30127-1-AP (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PARP14 Polyclonal antibody

PARP14 Polyclonal Antibody for IHC, ELISA
Cat No. 30127-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

BAL2, KIAA1268, PARP 14, PARP14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30127-1-AP targets PARP14 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP14 fusion protein Ag32409 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 14
Calculated molecular weight203kd
GenBank accession numberNM_017554
Gene Symbol PARP14
Gene ID (NCBI) 54625
RRIDAB_3086239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ460N5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

Protocols

Product Specific Protocols
IHC protocol for PARP14 antibody 30127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Pterosin B improves cognitive dysfunction by promoting microglia M1/M2 polarization through inhibiting Klf5/Parp14 pathway

Authors - Yan Zhang
View Article