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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP14 Recombinant antibody (84039-6-RR)

    WB analysis using 84039-6-RR (same clone as 84039-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84039-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PARP14 Recombinant antibody (84039-6-RR)

    IHC staining of mouse colon using 84039-6-RR (same clone as 84039-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84039-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84039-6-RR

    Affinity and Kinetic Characterization of 84039-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84039-6-RR against Human PARP14 were performed. The affinity constant is 0.685 nM.

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PARP14 Recombinant antibody, PBS Only

PARP14 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84039-6-PBS
Clone No.241078C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

PARP 14, KIAA1268, BAL2, 241078C11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84039-6-PBS targets PARP14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PARP14 fusion protein Ag35007 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 14
Calculated molecular weight203kd
Observed molecular weight200 kDa
GenBank accession numberNM_017554
Gene Symbol PARP14
Gene ID (NCBI) 54625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ460N5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).