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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PARP2 Polyclonal antibody

PARP2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20555-1-AP

Synonyms

ADPRT2, ADPRT-2, ADPRTL2, ARTD2, hPARP-2, pADPRT-2, PARP2, PARP-2, Poly [ADP-ribose] polymerase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP2 Polyclonal antibody (20555-1-AP)

    WB analysis using 20555-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PARP2 Polyclonal antibody (20555-1-AP)

    WB analysis of mouse testis using 20555-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20555-1-AP (PARP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of mouse brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of mouse brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of mouse testis using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of mouse testis using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of rat brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using PARP2 Polyclonal antibody (20555-1-AP)

    IHC staining of rat brain using 20555-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20555-1-AP (PARP2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive IHC detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20555-1-AP targets PARP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 2
Calculated molecular weight 66 kDa
Observed molecular weight60-66 kDa
GenBank accession numberNM_005484
Gene symbol PARP2
Gene ID (NCBI) 10038
RRIDAB_10694417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PRP2 catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor. The antibody is specific to PARP2 (C-terminal). PARP2 can be detected as about 62 kDa (PMID: 10364231).

Protocols

Product Specific Protocols
WB protocol for PARP2 antibody 20555-1-APDownload protocol
IHC protocol for PARP2 antibody 20555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols