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PARP2 Polyclonal antibody

PARP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55149-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

ADPRT 2, ADPRT2, ADPRTL2, ADPRTL3, hPARP 2, pADPRT 2, PARP 2, PARP2, poly (ADP ribose) polymerase 2, Poly [ADP ribose] polymerase 2, Poly[ADP ribose] synthase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 600 μg/ml
20 μL, 600 μg/ml
150 μL, 600 μg/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, U2OS cells, SH-SY5Y cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55149-1-AP targets PARP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase 2
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_005484
Gene Symbol PARP2
Gene ID (NCBI) 10038
RRIDAB_10858796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

Protocols

Product Specific Protocols
WB protocol for PARP2 antibody 55149-1-APDownload protocol
IF protocol for PARP2 antibody 55149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.

Authors - Xueying Wang
WB

Cells

Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development

Authors - Tatiana Kamaletdinova
humanWB

Eur J Med Chem

Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer.

Authors - Chunlan Pu
mouseWB

Int J Biol Sci

PARP1 Promotes Heart Regeneration and Cardiomyocyte Proliferation

Authors - Jiangcheng Shu
mouse,ratWB

EMBO J

Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function

Authors - Quanjiang Zhang
humanWB

Eur J Med Chem

Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation

Authors - Amandeep Thakur
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