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  • Featured Product
  • KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal antibody

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11202-1-AP

Synonyms

Actopaxin, Alpha parvin, Alpha Parvin/Actopaxin, CH ILKBP, MXRA2, PARVA, parvin, alpha, α-Parvin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Alpha Parvin/Actopaxin Polyclonal antibody (11202-1-AP)

    WB analysis of HeLa using 11202-1-AP

    WB result of α-Parvin/Actopaxin antibody (11202-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

  • Western Blot (WB) analysis of human liver tissue using Alpha Parvin/Actopaxin Polyclonal antibody (11202-1-AP)

    WB analysis of human liver using 11202-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Alpha Parvin/Actopaxin Polyclonal antibody (11202-1-AP)

    WB analysis of human kidney using 11202-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Alpha Parvin/Actopaxin Polyclonal antibody (11202-1-AP)

    WB analysis of Jurkat using 11202-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (11202-1-AP)

    IHC staining of human prostate cancer using 11202-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11202-1-AP (Actopaxin antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, HeLa cells, human kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11202-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Parvin/Actopaxin fusion protein Ag1694 相同性解析による交差性が予測される生物種
Full Name parvin, alpha
Calculated molecular weight 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC016713
Gene symbol Alpha Parvin/PARVA
Gene ID (NCBI) 55742
RRIDAB_2236617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Parvin/Actopaxin antibody 11202-1-APDownload protocol
IHC protocol for Alpha Parvin/Actopaxin antibody 11202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Androl Urol

Downregulation of PARVA promotes metastasis by modulating integrin-linked kinase activity and regulating MAPK/ERK and MLC2 signaling in prostate cancer.

Authors - Cong Huang
View Article
mouseWB

Cell Death Differ

Integrin-KCNB1 potassium channel complexes regulate neocortical neuronal development and are implicated in epilepsy

Authors - Alessandro Bortolami
View Article