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  • Featured Product
  • KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal antibody

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55268-1-AP

Synonyms

Alpha parvin, Actopaxin, PARVA, α-Parvin, CH-ILKBP

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of U2OS using 55268-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of HeLa using 55268-1-AP

    WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

  • Western Blot (WB) analysis of mouse kidney tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of mouse kidney using 55268-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of U2OS cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IP experiment of U2OS using 55268-1-AP

    IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human stomach cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 55268-1-AP (Alpha Parvin/Actopaxin antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human liver cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human liver cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human heart using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human heart using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IF Staining of MDCK using 55268-1-AP

    Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, mouse kidney tissue
Positive IP detected inU2OS cells
Positive IHC detected inhuman stomach cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55268-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name parvin, alpha
Calculated molecular weight 42 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_018222
Gene symbol Alpha Parvin/PARVA
Gene ID (NCBI) 55742
RRIDAB_10951112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Protocols

Product Specific Protocols
WB protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IHC protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IF protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IP protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
View Article
humanWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article