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  • Featured Product
  • KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal antibody

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55268-1-AP

Synonyms

Actopaxin, Alpha parvin, Alpha Parvin/Actopaxin, CH ILKBP, MXRA2, PARVA, parvin, alpha, α-Parvin

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of U2OS using 55268-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of HeLa using 55268-1-AP

    WB result of α-Parvin/Actopaxin antibody (55268-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

  • Western Blot (WB) analysis of mouse kidney tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    WB analysis of mouse kidney using 55268-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55268-1-AP (PARVA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of U2OS cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IP experiment of U2OS using 55268-1-AP

    IP result of anti-Alpha Parvin/Actopaxin (IP:55268-1-AP, 4ug; Detection:55268-1-AP 1:1000) with U2OS cells lysate 0 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human heart using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human heart using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55268-1-AP (PARVA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human liver cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IHC staining of human liver cancer using 55268-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55268-1-AP (PARVA Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Alpha Parvin/Actopaxin Polyclonal antibody (55268-1-AP)

    IF Staining of MDCK using 55268-1-AP

    Immunofluorescent analysis of MDCK cells using 55268-1-AP (PARVA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, mouse kidney tissue
Positive IP detected inU2OS cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55268-1-AP targets Alpha Parvin/Actopaxin in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name parvin, alpha
Calculated molecular weight 42 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_018222
Gene symbol Alpha Parvin/PARVA
Gene ID (NCBI) 55742
RRIDAB_10951112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

α-Parvin (PARVA), also named as MXRA2, Actopaxin and CH-ILKBP, belongs to the parvin family. It probably plays a role in the regulation of cell adhesion and cytoskeleton organization. (PMID:11134073) This antibody is specific to α-Parvin.

Protocols

Product Specific Protocols
WB protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IHC protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IF protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
IP protocol for Alpha Parvin/Actopaxin antibody 55268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,CoIP

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
View Article
humanWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
View Article