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PASK Polyclonal antibody

PASK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14396-1-AP

Synonyms

PASKIN, PAS kinase, PAS domain-containing serine/threonine-protein kinase, KIAA0135, hPASK

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PASK Polyclonal antibody (14396-1-AP)

    WB analysis of HEK-293 using 14396-1-AP

    WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PASK Polyclonal antibody (14396-1-AP)

    WB analysis using 14396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PASK Polyclonal antibody (14396-1-AP)

    WB analysis of K-562 using 14396-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PASK Polyclonal antibody (14396-1-AP)

    IP experiment of K-562 using 14396-1-AP

    IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of mouse testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human prostate hyperplasia using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human prostate hyperplasia using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PASK Polyclonal antibody (14396-1-AP)

    IHC staining of human testis using 14396-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PASK Polyclonal antibody (14396-1-AP)

    IF Staining of HeLa using 14396-1-AP

    Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, human prostate hyperplasia tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14396-1-AP targets PASK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PASK fusion protein Ag5893 相同性解析による交差性が予測される生物種
Full Name PAS domain containing serine/threonine kinase
Calculated molecular weight 143 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC063585
Gene symbol PASK
Gene ID (NCBI) 23178
RRIDAB_2878053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.

Protocols

Product Specific Protocols
WB protocol for PASK antibody 14396-1-APDownload protocol
IHC protocol for PASK antibody 14396-1-APDownload protocol
IF protocol for PASK antibody 14396-1-APDownload protocol
IP protocol for PASK antibody 14396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols