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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CPB1 Polyclonal antibody

CPB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12600-1-AP

Synonyms

Carboxypeptidase B, carboxypeptidase B1 (tissue), CPB, CPB1, Pancreas specific protein, PASP, PCPB

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    WB analysis of mouse pancreas using 12600-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    WB analysis of mouse pancreas using 12600-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    WB analysis of rat pancreas using 12600-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    WB analysis of mouse pancreas using 12600-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12600-1-AP (CPB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas cancer using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas cancer using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12600-1-AP (CPB1 antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human kidney using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human kidney using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CPB1 Polyclonal antibody (12600-1-AP)

    IHC staining of human pancreas using 12600-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12600-1-AP (CPB1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12600-1-AP targets CPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPB1 fusion protein Ag3243 相同性解析による交差性が予測される生物種
Full Name carboxypeptidase B1 (tissue)
Calculated molecular weight 417 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015338
Gene symbol CPB1
Gene ID (NCBI) 1360
RRIDAB_2261100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.

Protocols

Product Specific Protocols
WB protocol for CPB1 antibody 12600-1-APDownload protocol
IHC protocol for CPB1 antibody 12600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

Is Carboxypeptidase B1 a Prognostic Marker for Ductal Carcinoma In Situ?

Authors - Charu Kothari
View Article
humanWB,IHC

Transl Oncol

Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.

Authors - Yang Song
View Article
ratWB

Arch Gynecol Obstet

Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling.

Authors - Xiaohua Li
View Article