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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PATL1 Polyclonal antibody (21631-1-AP)

    WB analysis using 21631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21631-1-AP (PATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PATL1 Polyclonal antibody (21631-1-AP)

    WB analysis of HeLa using 21631-1-AP

    WB result of PATL1 antibody (21631-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PATL1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of K-562 cells using PATL1 Polyclonal antibody (21631-1-AP)

    IP experiment of K-562 using 21631-1-AP

    IP result of anti-PATL1 (IP:21631-1-AP, 4ug; Detection:21631-1-AP 1:300) with K-562 cells lysate 3320ug.

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  • KD/KO Validated

PATL1 Polyclonal antibody

PATL1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21631-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

PAT1 like protein 1, patellin1, PATL1, Protein PAT1 homolog 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, K-562 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21631-1-AP targets PATL1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PATL1 fusion protein Ag16247 相同性解析による交差性が予測される生物種
Full Name protein associated with topoisomerase II homolog 1 (yeast)
Calculated molecular weight 770 aa, 87 kDa
Observed molecular weight87 kDa
GenBank accession numberBC109038
Gene Symbol PATL1
Gene ID (NCBI) 219988
RRIDAB_2935454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Patellin1 (PATL1) is a human homolog to yeast P body component Pat1, localizes to P bodies and is required for P body formation. Furthermore, Pat1 is the only decapping activator protein that, besides interacting with deadenylated mRNAs, also binds to eIF4G-, eIF4E- and Pab1p-associated polyadenylated mRNA and locates to polysomes.

Protocols

Product Specific Protocols
WB protocol for PATL1 antibody 21631-1-APDownload protocol
IP protocol for PATL1 antibody 21631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols