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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of rat cerebellum using 67529-1-Ig (same clone as 67529-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of JAR cells using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of JAR using 67529-1-Ig (same clone as 67529-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of hTERT-RPE1 using 67529-1-Ig (same clone as 67529-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using PAX6 Monoclonal antibody (67529-1-Ig)

    WB analysis of Neuro-2a using 67529-1-Ig (same clone as 67529-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PAX6 Monoclonal antibody (67529-1-Ig)

    IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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PAX6 Monoclonal antibody, PBS Only

PAX6 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67529-1-PBS
Clone No.2C5A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, Indirect ELISA

Synonyms

Pax-6, PAX 6, Paired box protein Pax-6, Paired box protein Pax 6, paired box 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67529-1-PBS targets PAX6 in WB, IHC, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PAX6 fusion protein Ag29977 相同性解析による交差性が予測される生物種
Full Name paired box 6
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC011953
Gene Symbol PAX6
Gene ID (NCBI) 5080
RRIDAB_2882748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26367
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.