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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAX8 Monoclonal antibody, PBS Only

PAX8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B3

Cat no : 60145-4-PBS

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using PAX8 Monoclonal antibody (60145-4-Ig)

    WB analysis of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human thyroid gland tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    WB analysis of human thyroid gland using 60145-4-Ig (same clone as 60145-4-PBS)

    human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using PAX8 Monoclonal antibody (60145-4-Ig)

    IF Staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (60145-4-Ig, Clone: 4H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PAX8 Monoclonal antibody (60145-4-Ig)

    IF Staining of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

    Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60145-4-PBS targets PAX8 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_10643528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).