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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAX8 Recombinant antibody

PAX8 Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D20

Cat no : 80022-1-RR

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant antibody (80022-1-RR)

    IHC staining of human ovary tumor using 80022-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80022-1-RR (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Recombinant antibody (80022-1-RR)

    IHC staining of human ovary tumor using 80022-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80022-1-RR (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80022-1-RR targets PAX8 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PAX8 fusion protein Ag16019 相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 450 aa, 48 kDa
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

Protocols

Product Specific Protocols
IHC protocol for PAX8 antibody 80022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols