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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis of HeLa using 16110-1-AP (same clone as 16110-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis of mouse ovary using 16110-1-AP (same clone as 16110-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis of mouse testis using 16110-1-AP (same clone as 16110-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis of Jurkat using 16110-1-AP (same clone as 16110-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PBK/SPK Polyclonal antibody (16110-1-AP)

    WB analysis of NIH/3T3 using 16110-1-AP (same clone as 16110-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human lung cancer using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human skin cancer using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human skin cancer using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBK/SPK Polyclonal antibody (16110-1-AP)

    IHC staining of human lung cancer using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using PBK/SPK Polyclonal antibody (16110-1-AP)

    FC experiment of HepG2 using 16110-1-AP (same clone as 16110-1-PBS)

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PBK/SPK Polyclonal antibody, PBS Only

PBK/SPK Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 16110-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

PBK, MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16110-1-PBS targets PBK/SPK in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBK/SPK fusion protein Ag9033 相同性解析による交差性が予測される生物種
Full Name PDZ binding kinase
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC015191
Gene Symbol PBK
Gene ID (NCBI) 55872
RRIDAB_2283508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KB5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).