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- KD/KO Validated
CoraLite® Plus 488-conjugated PBK Polyclonal antibody
PBK Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-16110
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-16110 targets PBK in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PBK fusion protein Ag9033 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ binding kinase |
| Calculated molecular weight | 322 aa, 36 kDa |
| Observed molecular weight | 36-40 kDa |
| GenBank accession number | BC015191 |
| Gene symbol | PBK |
| Gene ID (NCBI) | 55872 |
| RRID | AB_2919131 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PBK antibody CL488-16110 | Download protocol |
| FC protocol for CL Plus 488 PBK antibody CL488-16110 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
