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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PBLD Polyclonal antibody

PBLD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27891-1-AP

Synonyms

MAWBP, MAWD binding protein, MAWDBP, PBLD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using PBLD Polyclonal antibody (27891-1-AP)

    WB analysis of mouse kidney using 27891-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PBLD Polyclonal antibody (27891-1-AP)

    WB analysis of mouse liver using 27891-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PBLD Polyclonal antibody (27891-1-AP)

    WB analysis of mouse kidney using 27891-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PBLD Polyclonal antibody (27891-1-AP)

    IHC staining of human kidney using 27891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PBLD Polyclonal antibody (27891-1-AP)

    IHC staining of human kidney using 27891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27891-1-AP targets PBLD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBLD fusion protein Ag23591 相同性解析による交差性が予測される生物種
Full Name phenazine biosynthesis-like protein domain containing
Observed molecular weight 30-32 kDa
GenBank accession numberBC009738
Gene symbol PBLD
Gene ID (NCBI) 64081
RRIDAB_2881007
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PBLD antibody 27891-1-APDownload protocol
IHC protocol for PBLD antibody 27891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols