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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using PBLD Monoclonal antibody (68317-1-Ig)

    WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using PBLD Monoclonal antibody (68317-1-Ig)

    WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PBLD Monoclonal antibody (68317-1-Ig)

    IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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PBLD Monoclonal antibody, PBS Only

PBLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68317-1-PBS
Clone No.1G11D4

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68317-1-PBS targets PBLD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PBLD fusion protein Ag23827 相同性解析による交差性が予測される生物種
Full Name phenazine biosynthesis-like protein domain containing
Observed molecular weight30 kDa
GenBank accession numberBC009738
Gene Symbol PBLD
Gene ID (NCBI) 64081
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30039
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)