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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PBRM1 Recombinant antibody (83677-2-RR)

    WB analysis using 83677-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83677-2-RR (PBRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBRM1 Recombinant antibody (83677-2-RR)

    IF Staining of HeLa using 83677-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBRM1 antibody (83677-2-RR, Clone: 240655H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83677-2-RR

    Affinity and Kinetic Characterization of 83677-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83677-2-RR against Human PBRM1 were performed. The affinity constant is 2.34 nM.

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PBRM1 Recombinant antibody

PBRM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83677-2-RR
Clone No.240655H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

PBRM 1, PB1, hPB1, BRG1-associated factor 180, BAF180

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HeLa cellls, U2OS cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83677-2-RR targets PBRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PBRM1 fusion protein Ag33623 相同性解析による交差性が予測される生物種
Full Name polybromo 1
Calculated molecular weight 180 kDa
Observed molecular weight180-195 kDa
GenBank accession numberBC015323
Gene Symbol PBRM1
Gene ID (NCBI) 55193
RRIDAB_3671280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86U86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.

Protocols

Product Specific Protocols
WB protocol for PBRM1 antibody 83677-2-RRDownload protocol
IF protocol for PBRM1 antibody 83677-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols