PBX1 Polyclonal antibody

Name: PBX1 Polyclonal antibody
Brand: Proteintech
SKU: 18204-1-AP

PBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18204-1-AP

Synonyms

PRL, Pre-B-cell leukemia transcription factor 1, pre B cell leukemia homeobox 1, Homeobox protein PRL, Homeobox protein PBX1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of A2780 using 18204-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18204-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 18204-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 18204-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A2780 using 18204-1-AP
IP result of anti-PBX1 (IP:18204-1-AP, 4ug; Detection:18204-1-AP 1:500) with A2780 cells lysate 1000ug.
IHC staining of rat kidney using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 18204-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18204-1-AP
Immunofluorescent analysis of HeLa cells using 18204-1-AP (PBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 18204-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A2780 cells, HeLa cells
Positive IP detected in	A2780 cells
Positive IHC detected in	human thyroid cancer tissue, human cervical cancer tissue, mouse brain tissue, mouse kidney tissue, mouse pancreas tissue, rat brain tissue, rat kidney tissue, rat pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 3 publications below
IF	See 3 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

18204-1-AP targets PBX1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PBX1 fusion protein Ag12817 相同性解析による交差性が予測される生物種
Full Name	pre-B-cell leukemia homeobox 1
Calculated molecular weight	430 aa, 47 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC101578
Gene symbol	PBX1
Gene ID (NCBI)	5087
RRID	AB_10951860
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b) and molecular weight of PBX1 is 47 kDa and 38 kDa.

Protocols

Product Specific Protocols
WB protocol for PBX1 antibody 18204-1-AP	Download protocol
IHC protocol for PBX1 antibody 18204-1-AP	Download protocol
IF protocol for PBX1 antibody 18204-1-AP	Download protocol
IP protocol for PBX1 antibody 18204-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human,rat	WB,IF	Int J Oral Sci Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis Authors - Xiao Yang View Article
mouse	WB	J Neurochem Decreased levels of constitutive proteasomes in experimental autoimmune encephalomyelitis may be caused by a combination of subunit displacement and reduced Nfe2l1 expression. Authors - Kara L Shanley View Article
mouse	WB	Neuroscience Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion. Authors - Zhengfang Lu View Article
human	WB	Cell Death Dis M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops Authors - Hongda Lin View Article
mouse	ChIP	Cell Mol Life Sci Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility Authors - Fei-Chen Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PBX1 Polyclonal antibody

PBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A2780 using 18204-1-AP

WB analysis of HeLa using 18204-1-AP

WB analysis of A2780 using 18204-1-AP

WB analysis of A2780 using 18204-1-AP

IP experiment of A2780 using 18204-1-AP

IHC staining of rat kidney using 18204-1-AP

IHC staining of rat pancreas using 18204-1-AP

IHC staining of mouse kidney using 18204-1-AP

IHC staining of human cervical cancer using 18204-1-AP

IHC staining of human thyroid cancer using 18204-1-AP

IHC staining of human thyroid cancer using 18204-1-AP

IHC staining of mouse brain using 18204-1-AP

IHC staining of mouse pancreas using 18204-1-AP

IHC staining of rat brain using 18204-1-AP

IF Staining of HeLa using 18204-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis

Decreased levels of constitutive proteasomes in experimental autoimmune encephalomyelitis may be caused by a combination of subunit displacement and reduced Nfe2l1 expression.

Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.

M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops

Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility