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  • Featured Product
  • KD/KO Validated

PBX3 Polyclonal antibody, PBS Only

PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12571-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using PBX3 Polyclonal antibody (12571-1-AP)

    WB analysis of human lung using 12571-1-AP (same clone as 12571-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PBX3 Polyclonal antibody (12571-1-AP)

    IP experiment of HL-60 using 12571-1-AP (same clone as 12571-1-PBS)

    IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBX3 Polyclonal antibody (12571-1-AP)

    IHC staining of human lung cancer using 12571-1-AP (same clone as 12571-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBX3 Polyclonal antibody (12571-1-AP)

    IF Staining of HeLa using 12571-1-AP (same clone as 12571-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12571-1-AP (PBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12571-1-PBS targets PBX3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBX3 fusion protein Ag3239 相同性解析による交差性が予測される生物種
Full Name pre-B-cell leukemia homeobox 3
Calculated molecular weight 434 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC016977
Gene symbol PBX3
Gene ID (NCBI) 5090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PBX3, also named as Pre-B-cell leukemia transcription factor 3, is a 434 amino acid protein ,which contains 1 homeobox DNA-binding domain and belongs to the TALE/PBX homeobox family. PBX3 localizes in the nucleus and as a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. PBX3 is ubiquitously expressed in many tissues.