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  • Featured Product
  • KD/KO Validated

PBX3 Polyclonal antibody, PBS Only

PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12571-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using PBX3 Polyclonal antibody (12571-1-AP)

    WB analysis of human lung using 12571-1-AP (same clone as 12571-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PBX3 Polyclonal antibody (12571-1-AP)

    IP experiment of HL-60 using 12571-1-AP (same clone as 12571-1-PBS)

    IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBX3 Polyclonal antibody (12571-1-AP)

    IHC staining of human lung cancer using 12571-1-AP (same clone as 12571-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBX3 Polyclonal antibody (12571-1-AP)

    IF Staining of HeLa using 12571-1-AP (same clone as 12571-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12571-1-AP (PBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12571-1-PBS targets PBX3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBX3 fusion protein Ag3239 相同性解析による交差性が予測される生物種
Full Name pre-B-cell leukemia homeobox 3
Calculated molecular weight 434 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC016977
Gene symbol PBX3
Gene ID (NCBI) 5090
RRIDAB_2160469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PBX3, also named as Pre-B-cell leukemia transcription factor 3, is a 434 amino acid protein ,which contains 1 homeobox DNA-binding domain and belongs to the TALE/PBX homeobox family. PBX3 localizes in the nucleus and as a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. PBX3 is ubiquitously expressed in many tissues.