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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PBX4 Polyclonal antibody

PBX4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22323-1-AP

Synonyms

Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PBX4 Polyclonal antibody (22323-1-AP)

    WB analysis of HEK-293 using 22323-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PBX4 Polyclonal antibody (22323-1-AP)

    WB analysis of COLO 320 using 22323-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PBX4 Polyclonal antibody (22323-1-AP)

    WB analysis of Jurkat using 22323-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using PBX4 Polyclonal antibody (22323-1-AP)

    WB analysis of A2780 using 22323-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using PBX4 Polyclonal antibody (22323-1-AP)

    WB analysis of SKOV-3 using 22323-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A2780 cells using PBX4 Polyclonal antibody (22323-1-AP)

    IP experiment of A2780 using 22323-1-AP

    IP result of anti-PBX4 (IP:22323-1-AP, 4ug; Detection:22323-1-AP 1:300) with A2780 cells lysate 3600 ug.

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Tested Applications

Positive WB detected inHEK-293 cells, SKOV-3 cells, A2780 cells, COLO 320 cells, Jurkat cells
Positive IP detected inA2780 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22323-1-AP is PBX4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBX4 fusion protein Ag17768 相同性解析による交差性が予測される生物種
Full Name pre-B-cell leukemia homeobox 4
Calculated molecular weight 374 aa, 41 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC141859
Gene symbol PBX4
Gene ID (NCBI) 80714
RRIDAB_2879071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pre-B-cell leukemia homeobox 4 (PBX4), also named as Homeobox protein PBX4, is one of the pbx gene encode homeodomain containing transcriptional regulators that interact with other proteins to control embryogenesis and tumorigenesis. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase. PBX4 may form heterodimers with other homeobox genes (hoxb1b or meis3) to specify different developmental fates during vertebrate embryogenesis (PMID:10679934). The trimeric complex containing Pbx4, Meis3, and Hoxb1b was also formed. The calculated molecular weight of PBX4 is about 41 kDa, but the molecular weight of heterodimers (PBX4 and hoxb1b) is about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for PBX4 antibody 22323-1-APDownload protocol
IP protocol for PBX4 antibody 22323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols