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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    WB analysis of BxPC-3 using 12102-1-AP (same clone as 12102-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    WB analysis of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    WB analysis of A549 using 12102-1-AP (same clone as 12102-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    IP experiment of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

    IP result of anti-PBXIP1 (IP:12102-1-AP, 3ug; Detection:12102-1-AP 1:800) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBXIP1 Polyclonal antibody (12102-1-AP)

    IHC staining of human lung cancer using 12102-1-AP (same clone as 12102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PBXIP1 Polyclonal antibody (12102-1-AP)

    IHC staining of human lung cancer using 12102-1-AP (same clone as 12102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    IF Staining of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PBXIP1 Polyclonal antibody (12102-1-AP)

    IF Staining of HepG2 using 12102-1-AP (same clone as 12102-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PBXIP1 Polyclonal antibody, PBS Only

PBXIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12102-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12102-1-PBS targets PBXIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PBXIP1 fusion protein Ag2746 相同性解析による交差性が予測される生物種
Full Name pre-B-cell leukemia homeobox interacting protein 1
Calculated molecular weight 731 aa, 81 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC016852
Gene Symbol PBXIP1
Gene ID (NCBI) 57326
RRIDAB_2160484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AQ6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).