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Pyruvate Carboxylase Polyclonal antibody

Name: Pyruvate Carboxylase Polyclonal antibody
Brand: Proteintech
SKU: 16588-1-AP
Rating: 5.00 (1 reviews)

Pyruvate Carboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 16588-1-AP

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 16588-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16588-1-AP
WB result of Pyruvate Carboxylase antibody (16588-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.
IP experiment of HepG2 using 16588-1-AP
IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:1000) with HepG2 cells lysate 2640 ug.
IP experiment of mouse liver using 16588-1-AP
IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.
IHC staining of human liver cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16588-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16588-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
IF Staining of HepG2 using 16588-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16588-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected in	HepG2 cells, mouse liver tissue
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 34 publications below
IHC	See 5 publications below
IF	See 4 publications below
IP	See 3 publications below

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Pyruvate Carboxylase fusion protein Ag8857 相同性解析による交差性が予測される生物種
Full Name	pyruvate carboxylase
Calculated molecular weight	1178 aa, 130 kDa
Observed molecular weight	125-130 kDa
GenBank accession number	BC011617
Gene symbol	Pyruvate Carboxylase
Gene ID (NCBI)	5091
RRID	AB_1851513
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Protocols

Product Specific Protocols
WB protocol for Pyruvate Carboxylase antibody 16588-1-AP	Download protocol
IHC protocol for Pyruvate Carboxylase antibody 16588-1-AP	Download protocol
IF protocol for Pyruvate Carboxylase antibody 16588-1-AP	Download protocol
IP protocol for Pyruvate Carboxylase antibody 16588-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells Authors - Kevin M Rattigan View Article
human	WB	Nat Commun SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition. Authors - Peter J Mullen View Article
mouse	WB	Nat Commun Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise. Authors - Jin-Ho Koh View Article
human	IP	Nat Commun The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase. Authors - Alastair G Kerr View Article
mouse	WB	Cancer Res A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma. Authors - Jennifer G Gill View Article
mouse	WB	Elife Differential requirements for mitochondrial electron transport chain components in the adult murine liver Authors - Nicholas P Lesner View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Kevin (Verified Customer) (02-10-2022)

This worked as well as other much more expensive antibodies from differenet suppliers

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: K562

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Pyruvate Carboxylase Polyclonal antibody

Pyruvate Carboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 16588-1-AP

WB analysis of HepG2 using 16588-1-AP

IP experiment of HepG2 using 16588-1-AP

IP experiment of mouse liver using 16588-1-AP

IHC staining of human liver cancer using 16588-1-AP

IHC staining of human liver cancer using 16588-1-AP

IHC staining of human liver cancer using 16588-1-AP

IHC staining of human breast cancer using 16588-1-AP

IHC staining of human breast cancer using 16588-1-AP

IHC staining of human liver cancer using 16588-1-AP

IF Staining of HepG2 using 16588-1-AP

IF Staining of HepG2 using 16588-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells

SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.

A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.

Differential requirements for mitochondrial electron transport chain components in the adult murine liver

Reviews