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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCBP3 Polyclonal antibody (16141-1-AP)

    WB analysis using 16141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16141-1-AP (PCBP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCBP3 Polyclonal antibody (16141-1-AP)

    IF Staining of MCF-7 using 16141-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PCBP3 antibody (16141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PCBP3 Polyclonal antibody

PCBP3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16141-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCBP3 fusion protein Ag9202 相同性解析による交差性が予測される生物種
Full Name poly(rC) binding protein 3
Calculated molecular weight 313 aa, 33 kDa
Observed molecular weight37 kDa
GenBank accession numberBC012061
Gene Symbol PCBP3
Gene ID (NCBI) 54039
RRIDAB_3085481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57721
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.

Protocols

Product Specific Protocols
WB protocol for PCBP3 antibody 16141-1-APDownload protocol
IF protocol for PCBP3 antibody 16141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols