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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCBP3 Polyclonal antibody

PCBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16141-1-AP

Synonyms

PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCBP3 Polyclonal antibody (16141-1-AP)

    WB analysis using 16141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16141-1-AP (PCBP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCBP3 Polyclonal antibody (16141-1-AP)

    IF Staining of MCF-7 using 16141-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PCBP3 antibody (16141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHT-1080 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16141-1-AP targets PCBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCBP3 fusion protein Ag9202 相同性解析による交差性が予測される生物種
Full Name poly(rC) binding protein 3
Calculated molecular weight 313 aa, 33 kDa
Observed molecular weight37 kDa
GenBank accession numberBC012061
Gene symbol PCBP3
Gene ID (NCBI) 54039
RRIDAB_3085481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions.

Protocols

Product Specific Protocols
WB protocol for PCBP3 antibody 16141-1-APDownload protocol
IF protocol for PCBP3 antibody 16141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols