PCCB Polyclonal antibody

PCCB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11139-1-AP

Synonyms

Propionyl-CoA carboxylase beta chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCase subunit beta, EC:6.4.1.3


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Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11139-1-AP targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCCB fusion protein Ag1608 相同性解析による交差性が予測される生物種
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
RRIDAB_2877746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCCB antibody 11139-1-APDownload protocol
IHC protocol for PCCB antibody 11139-1-APDownload protocol
IF protocol for PCCB antibody 11139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
humanWB

Biochim Biophys Acta Mol Basis Dis

ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis

Authors - Lan Wang
humanIHC

Am J Pathol

Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis

Authors - Kai Xu
WB,IF

Proc Natl Acad Sci U S A

Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues

Authors - Jie Hou