Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11139-1-AP targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCCB fusion protein Ag1608 相同性解析による交差性が予測される生物種 |
| Full Name | propionyl Coenzyme A carboxylase, beta polypeptide |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC013768 |
| Gene Symbol | PCCB |
| Gene ID (NCBI) | 5096 |
| RRID | AB_2877746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCCB antibody 11139-1-AP | Download protocol |
| IHC protocol for PCCB antibody 11139-1-AP | Download protocol |
| IF protocol for PCCB antibody 11139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. | ||
Biochim Biophys Acta Mol Basis Dis ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis | ||
Am J Pathol Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis | ||
Proc Natl Acad Sci U S A Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues |