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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDH1 Polyclonal antibody

PCDH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13645-1-AP

Synonyms

Protocadherin-42, Protocadherin-1, PC42, Cadherin-like protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PCDH1 Polyclonal antibody (13645-1-AP)

    WB analysis of fetal human brain using 13645-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13645-1-AP (PCDH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCDH1 Polyclonal antibody (13645-1-AP)

    WB analysis of human brain using 13645-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13645-1-AP (PCDH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCDH1 Polyclonal antibody (13645-1-AP)

    WB analysis of human brain using 13645-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13645-1-AP (PCDH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PCDH1 Polyclonal antibody (13645-1-AP)

    IHC staining of human stomach using 13645-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13645-1-AP (PCDH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13645-1-AP targets PCDH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDH1 fusion protein Ag4591 相同性解析による交差性が予測される生物種
Full Name protocadherin 1
Calculated molecular weight 1060 aa, 115 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC035812
Gene symbol PCDH1
Gene ID (NCBI) 5097
RRIDAB_2283373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCDH1 antibody 13645-1-APDownload protocol
IHC protocol for PCDH1 antibody 13645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nanomedicine

VEGF loaded nanofiber membranes inhibit chronic cerebral hypoperfusion-induced cognitive dysfunction by promoting HIF-1a/VEGF mediated angiogenesis

Authors - Yi-Fang Wu
View Article