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PCDH10 Polyclonal antibody
PCDH10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 21859-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
21859-1-AP targets PCDH10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCDH10 fusion protein Ag16494 相同性解析による交差性が予測される生物種 |
| Full Name | protocadherin 10 |
| Calculated molecular weight | 1040 aa, 113 kDa |
| Observed molecular weight | 113 kDa |
| GenBank accession number | BC111560 |
| Gene symbol | PCDH10 |
| Gene ID (NCBI) | 57575 |
| RRID | AB_2878929 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDH10 antibody 21859-1-AP | Download protocol |
| IHC protocol for PCDH10 antibody 21859-1-AP | Download protocol |
| IF protocol for PCDH10 antibody 21859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics A Compound AC1Q3QWB Selectively Disrupts HOTAIR-Mediated Recruitment of PRC2 and Enhances Cancer Therapy of DZNep. | ||
Clin Transl Med Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR-576-5p/PCDH10 axis. | ||
Int J Biol Sci FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sampath (Verified Customer) (03-11-2020) | Pcdh10 antibody worked very well for me. I saw the bands even after 5 sec of exposure at dilution 1:1000 o/n incubation.
 |
FH Aurelie (Verified Customer) (06-13-2019) | In tissue, there is some background but the antibody give a correct signal. I also try in human primary fibroblasts, the background is lower and the band at 130kd observed here , is not present.- dilution 1/1500 should be enough
 |
