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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDH8 Polyclonal antibody

PCDH8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14000-1-AP

Synonyms

Protocadherin-8, Arcadlin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP

    mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, Neuro-2a cells, U-251 cells, U-87 MG cells, unboiled mouse brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14000-1-AP targets PCDH8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDH8 fusion protein Ag5090 相同性解析による交差性が予測される生物種
Full Name protocadherin 8
Calculated molecular weight 1070 aa, 113 kDa
Observed molecular weight104 kDa,113 kDa
GenBank accession numberBC036025
Gene symbol PCDH8
Gene ID (NCBI) 5100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for PCDH8 antibody 14000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols