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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDH8 Polyclonal antibody

PCDH8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14000-1-AP

Synonyms

Protocadherin-8, Arcadlin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP

    mouse brain and unboiled mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCDH8 Polyclonal antibody (14000-1-AP)

    WB analysis using 14000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14000-1-AP (PCDH8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, Neuro-2a cells, U-251 cells, U-87 MG cells, unboiled mouse brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14000-1-AP targets PCDH8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDH8 fusion protein Ag5090 相同性解析による交差性が予測される生物種
Full Name protocadherin 8
Calculated molecular weight 1070 aa, 113 kDa
Observed molecular weight104 kDa,113 kDa
GenBank accession numberBC036025
Gene symbol PCDH8
Gene ID (NCBI) 5100
RRIDAB_3669175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for PCDH8 antibody 14000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols