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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDH8 Recombinant antibody (84134-3-RR)

    WB analysis using 84134-3-RR (same clone as 84134-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84134-3-RR (PCDH8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84134-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01033-1

    Cytometric bead array standard curve of MP01033-1

    Cytometric bead array standard curve of MP01033-1, PCDH8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84134-4-PBS. Detection antibody: 84134-3-PBS. Standard: Ag5090. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01033-2

    Cytometric bead array standard curve of MP01033-2

    Cytometric bead array standard curve of MP01033-2, PCDH8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84134-1-PBS. Detection antibody: 84134-3-PBS. Standard: Ag5090. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01033-3

    Cytometric bead array standard curve of MP01033-3

    Cytometric bead array standard curve of MP01033-3, PCDH8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84134-2-PBS. Detection antibody: 84134-3-PBS. Standard: Ag5090. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84134-3-RR

    Affinity and Kinetic Characterization of 84134-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84134-3-RR against Human PCDH8 were performed. The affinity constant is 0.633 nM.

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PCDH8 Recombinant antibody, PBS Only (Detector)

PCDH8 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84134-3-PBS
Clone No.241362C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Protocadherin-8, Arcadlin, 241362C6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84134-3-PBS targets PCDH8 as part of a matched antibody pair:

MP01033-1: 84134-4-PBS capture and 84134-3-PBS detection (validated in Cytometric bead array)

MP01033-2: 84134-1-PBS capture and 84134-3-PBS detection (validated in Cytometric bead array)

MP01033-3: 84134-2-PBS capture and 84134-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCDH8 fusion protein Ag5090 相同性解析による交差性が予測される生物種
Full Name protocadherin 8
Calculated molecular weight 1070 aa, 113 kDa
Observed molecular weight104-120 kDa
GenBank accession numberBC036025
Gene Symbol PCDH8
Gene ID (NCBI) 5100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95206
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Protocadherin-8 (PCDH8) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH8 is a tumor suppressor involved in tumorigenesis.