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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDHA2 Polyclonal antibody

PCDHA2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10127-2-AP

Synonyms

PCDH alpha 2, PCDH ALPHA2, PCDHA2, protocadherin alpha 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis using 10127-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of Raji using 10127-2-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of human heart using 10127-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of HeLa using 10127-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCDHA2 Polyclonal antibody (10127-2-AP)

    WB analysis of human brain using 10127-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PCDHA2 Polyclonal antibody (10127-2-AP)

    IP experiment of HeLa using 10127-2-AP

    IP result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug.

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Tested Applications

Positive WB detected inmouse brain tissue, Raji cells, human heart tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10127-2-AP targets PCDHA2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHA2 fusion protein Ag0173 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 2
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa, 80 kDa
GenBank accession numberBC003126
Gene symbol PCDHA2
Gene ID (NCBI) 56146
RRIDAB_2158964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).

Protocols

Product Specific Protocols
WB protocol for PCDHA2 antibody 10127-2-APDownload protocol
IP protocol for PCDHA2 antibody 10127-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols