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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDHA3 Polyclonal antibody

PCDHA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18803-1-AP

Synonyms

Protocadherin alpha-3, PCDH-alpha-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA3 Polyclonal antibody (18803-1-AP)

    WB analysis using 18803-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    WB analysis of mouse colon using 18803-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of mouse brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of human brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA3 Polyclonal antibody (18803-1-AP)

    IHC staining of human brain using 18803-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, mouse colon tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18803-1-AP targets PCDHA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 3
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC129807
Gene symbol PCDHA3
Gene ID (NCBI) 56145
RRIDAB_10596786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.

Protocols

Product Specific Protocols
WB protocol for PCDHA3 antibody 18803-1-APDownload protocol
IHC protocol for PCDHA3 antibody 18803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols