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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDHA5 Polyclonal antibody, PBS Only

PCDHA5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15270-1-PBS

Synonyms

PCDH ALPHA5, PCDH alpha 5, CRNR6, CNRS6, CNRN6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHA5 Polyclonal antibody (15270-1-AP)

    WB analysis using 15270-1-AP (same clone as 15270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    WB analysis of human brain using 15270-1-AP (same clone as 15270-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human brain using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human brain using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human gliomas using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human gliomas using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of rat cerebellum using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of rat cerebellum using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of mouse cerebellum using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human lung using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PCDHA5 Polyclonal antibody (15270-1-AP)

    IHC staining of human lung using 15270-1-AP (same clone as 15270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15270-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15270-1-PBS targets PCDHA5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHA5 fusion protein Ag7376 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 5
Calculated molecular weight 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC033735
Gene symbol PCDHA5
Gene ID (NCBI) 56143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.