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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDHA6 Polyclonal antibody

PCDHA6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12853-1-AP

Synonyms

CNR2, CNRN2, CNRS2, CRNR2, PCDH alpha 6, PCDH ALPHA6, PCDHA6, protocadherin alpha 6

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    WB analysis of rat brain using 12853-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCDHA6 Polyclonal antibody (12853-1-AP)

    WB analysis using 12853-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    WB analysis of human brain using 12853-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    WB analysis of mouse brain using 12853-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12853-1-AP (PCDHA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of mouse cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of mouse cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of human gliomas using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of human gliomas using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of rat cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PCDHA6 Polyclonal antibody (12853-1-AP)

    IHC staining of rat cerebellum using 12853-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12853-1-AP (PCDHA6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, human gliomas tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12853-1-AP targets PCDHA6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHA6 fusion protein Ag3493 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha 6
Calculated molecular weight 103 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC036674
Gene symbol PCDHA6
Gene ID (NCBI) 56142
RRIDAB_10642141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCDHA6 antibody 12853-1-APDownload protocol
IHC protocol for PCDHA6 antibody 12853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols