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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCDHAC1 Polyclonal antibody (24314-1-AP)

    WB analysis using 24314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24314-1-AP (PCDHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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PCDHAC1 Polyclonal antibody

PCDHAC1 Polyclonal Antibody for WB, ELISA
Cat No. 24314-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PCDH ALPHA C1, PCDHAC1, Protocadherin alpha C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, K-562 cells, SK-N-SH cells, SH-SY5Y cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24314-1-AP targets PCDHAC1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHAC1 fusion protein Ag19431 相同性解析による交差性が予測される生物種
Full Name protocadherin alpha subfamily C, 1
Calculated molecular weight 818 aa, 88 kDa
Observed molecular weight~110 kDa
GenBank accession numberBC136774
Gene Symbol PCDHAC1
Gene ID (NCBI) 56135
RRIDAB_3085730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHAC1 belongs to the α-protocadherin (PCDHA) cluster. The differential expression of PCDHAC1 may be related to the influence of maternal cadmium on fetal growth (PMID: 27544570).

Protocols

Product Specific Protocols
WB protocol for PCDHAC1 antibody 24314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols