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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCDHGB3 Polyclonal antibody

PCDHGB3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21204-1-AP

Synonyms

Protocadherin gamma-B3, PCDH-gamma-B3

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PCDHGB3 Polyclonal antibody (21204-1-AP)

    WB analysis of human brain using 21204-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21204-1-AP (PCDHGB3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using PCDHGB3 Polyclonal antibody (21204-1-AP)

    WB analysis of Neuro-2a using 21204-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21204-1-AP (PCDHGB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCDHGB3 Polyclonal antibody (21204-1-AP)

    IF Staining of HeLa using 21204-1-AP

    Immunofluorescent analysis of Hela cells, using PCDHGB3 antibody 21204-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman brain tissue, Neuro-2a cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21204-1-AP targets PCDHGB3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDHGB3 fusion protein Ag15569 相同性解析による交差性が予測される生物種
Full Name protocadherin gamma subfamily B, 3
Calculated molecular weight 929 aa, 101 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC150172
Gene symbol PCDHGB3
Gene ID (NCBI) 56102
RRIDAB_10858620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCDHGB3 antibody 21204-1-APDownload protocol
IF protocol for PCDHGB3 antibody 21204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols