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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCGF1 Polyclonal antibody (15581-1-AP)

    WB analysis using 15581-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15581-1-AP (PCGF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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PCGF1 Polyclonal antibody

PCGF1 Polyclonal Antibody for WB, ELISA
Cat No. 15581-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RNF3A 2, RING finger protein 68, polycomb group ring finger 1, NSPc1, Nervous system Polycomb-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15581-1-AP targets PCGF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCGF1 fusion protein Ag7983 相同性解析による交差性が予測される生物種
Full Name polycomb group ring finger 1
Calculated molecular weight 30 kDa
Observed molecular weight30-31 kDa
GenBank accession numberBC004952
Gene Symbol PCGF1
Gene ID (NCBI) 84759
RRIDAB_3669212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSM1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCGF1 (Polycomb group RING finger protein 1), also known as NSPC1. It is expected to be located in the nucleus. Human and mouse NSPc1 genes encode proteins with an N-terminal RING finger domain and share homology with Drosophila melanogaster lethal(3)73Ah and the mammalian Mel18 and Bmi1 genes. Transcripts are observed at 10 dpc in the otic vesicle, urogenital bud and dorsal root ganglia. At 11.5 dpc, transcripts are present in a subset of neural crest cell derivatives of the peripheral nervous system, and in the neural tube. NSPc1 expression is ubiquitous in adult tissue (PMID: 11287196). The molecular weight of PCGF1is 30 kDa.

Protocols

Product Specific Protocols
WB protocol for PCGF1 antibody 15581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols