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PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16082-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRamos cells, HepG2 cells, Jurkat cells, HEK-293T cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16082-1-AP targets PCIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCIF1 fusion protein Ag9080 相同性解析による交差性が予測される生物種
Full Name PDX1 C-terminal inhibiting factor 1
Calculated molecular weight 704 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC013365
Gene Symbol PCIF1
Gene ID (NCBI) 63935
RRIDAB_2159996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4Z3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCIF1 antibody 16082-1-APDownload protocol
IHC protocol for PCIF1 antibody 16082-1-APDownload protocol
IF protocol for PCIF1 antibody 16082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
humanWB

Nat Commun

HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1

Authors - Qiong Zhang
pigWB

Cell Rep

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.

Authors - Robert J J Jansens
human,mouseWB,IF

EMBO J

Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy

Authors - Lingling Wang
humanWB

Proc Natl Acad Sci U S A

PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection

Authors - Lingling Wang
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