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  • KD/KO Validated

PCK1 Polyclonal antibody

PCK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16754-1-AP

Synonyms

Serine-protein kinase PCK1, PEPCK-C, PEPCK1, EC:4.1.1.32, EC:2.7.11.-


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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16754-1-AP targets PCK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCK1 fusion protein Ag10261 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Calculated molecular weight 622 aa, 69 kDa
Observed molecular weight 66-69 kDa
GenBank accession numberBC023978
Gene symbol PCK1
Gene ID (NCBI) 5105
RRIDAB_2160031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Protocols

Product Specific Protocols
WB protocol for PCK1 antibody 16754-1-APDownload protocol
IF protocol for PCK1 antibody 16754-1-APDownload protocol
IP protocol for PCK1 antibody 16754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Authors - Daqian Xu
  • KD Validated
mouseWB

Nat Commun

Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.

Authors - Jingwei Ma
mouseIF

Adv Healthc Mater

Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment.

Authors - Jingjing Gan
humanWB

Biomaterials

Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.

Authors - Meiling Wu
mouseWB

Proc Natl Acad Sci U S A

Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.

Authors - Daniel Mauvoisin
  • KO Validated
mouseWB

Diabetes

Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity.

Authors - Yuanyang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (11-18-2022)

Detection of PCK1 in liver protein extracts

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
PCK1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:16754-1-AP)