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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCK2 Monoclonal antibody, PBS Only

PCK2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3F1

Cat no : 67676-1-PBS

Synonyms

PCK2, PEPCK, PEPCK M, PEPCK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCK2 Monoclonal antibody (67676-1-Ig)

    WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    WB analysis of rat liver using 67676-1-Ig (same clone as 67676-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    WB analysis of pig liver using 67676-1-Ig (same clone as 67676-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit liver tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    WB analysis of rabbit liver using 67676-1-Ig (same clone as 67676-1-PBS)

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PCK2 Monoclonal antibody (67676-1-Ig)

    IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67676-1-PBS targets PCK2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCK2 fusion protein Ag7021 相同性解析による交差性が予測される生物種
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Calculated molecular weight 71 kDa
Observed molecular weight68 kDa
GenBank accession numberBC001454
Gene symbol PCK2
Gene ID (NCBI) 5106
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.