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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCM1 Recombinant antibody

PCM1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242199A9

Cat no : 84979-1-RR

Synonyms

pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCM1 Recombinant antibody (84979-1-RR)

    WB analysis using 84979-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCM1 Recombinant antibody (84979-1-RR)

    WB analysis using 84979-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCM1 Recombinant antibody (84979-1-RR)

    IHC staining of human tonsillitis using 84979-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCM1 Recombinant antibody (84979-1-RR)

    IHC staining of human tonsillitis using 84979-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCM1 Recombinant antibody (84979-1-RR)

    IF Staining of HepG2 using 84979-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-1-RR, Clone: 242199A9 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using PCM1 Recombinant antibody (84979-1-RR)

    FC experiment of A549 using 84979-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of K562 using PCM1 Recombinant antibody (84979-1-RR)

    FC experiment of K562 using 84979-1-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84979-1-RR

    Affinity and Kinetic Characterization of 84979-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84979-1-RR against Human PCM1 were performed. The affinity constant is 21.0 nM.

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, A431 cells, U-251 cells, HT-1080 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK562, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84979-1-RR targets PCM1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCM1 fusion protein Ag13977 相同性解析による交差性が予測される生物種
Full Name pericentriolar material 1
Calculated molecular weight 2024 aa, 228 kDa
Observed molecular weight245-320 kDa
GenBank accession numberBC000453
Gene symbol PCM1
Gene ID (NCBI) 5108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Protocols

Product Specific Protocols
WB protocol for PCM1 antibody 84979-1-RRDownload protocol
IHC protocol for PCM1 antibody 84979-1-RRDownload protocol
IF protocol for PCM1 antibody 84979-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols