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KD/KO Validated

PCMT1 Polyclonal antibody

Name: PCMT1 Polyclonal antibody
Brand: Proteintech
SKU: 10519-1-AP

PCMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10519-1-AP

Synonyms

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 10519-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10519-1-AP
WB result of PCMT1 antibody (10519-1-AP, 1:1000) with si-Control and si-PCMT1 transfected HeLa cells.
WB analysis of HEK-293 using 10519-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10519-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 10519-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10519-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 10519-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 10519-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 10519-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10519-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa, HeLa cells, human testis tissue, HEK-293 cells, Raji cells, mouse testis tissue, rat testis tissue
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 2 publications below

Product Information

10519-1-AP targets PCMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PCMT1 fusion protein Ag0811 相同性解析による交差性が予測される生物種
Full Name	protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated molecular weight	25 kDa
Observed molecular weight	25-28 kDa
GenBank accession number	BC008748
Gene symbol	PCMT1
Gene ID (NCBI)	5110
RRID	AB_2160201
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Protocols

Product Specific Protocols
WB protocol for PCMT1 antibody 10519-1-AP	Download protocol
IHC protocol for PCMT1 antibody 10519-1-AP	Download protocol
IF protocol for PCMT1 antibody 10519-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Diabetologia Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase? Authors - W?gner A M AM View Article
mouse	WB	Int J Cardiol Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1. Authors - Yan Guijun G View Article
human	WB	Front Cell Dev Biol LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1. Authors - Li-Ming Dong View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article
human	IHC	Transl Cancer Res Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer Authors - Liyun Shan View Article
human	WB	J Exp Clin Cancer Res Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis. Authors - Jingjing Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCMT1 Polyclonal antibody

PCMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 10519-1-AP

WB analysis of HeLa using 10519-1-AP

WB analysis of HEK-293 using 10519-1-AP

WB analysis of Raji using 10519-1-AP

WB analysis of human testis using 10519-1-AP

WB analysis of HeLa using 10519-1-AP

IHC staining of human pancreas using 10519-1-AP

IHC staining of human pancreas using 10519-1-AP

IF Staining of HEK-293 using 10519-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?

Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.

LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer

Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.