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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCMT1 Monoclonal antibody, PBS Only

PCMT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C8G8

Cat no : 60172-1-PBS

Synonyms

PCMT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis using 60172-1-Ig (same clone as 60172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of pig brain using 60172-1-Ig (same clone as 60172-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of Raji using 60172-1-Ig (same clone as 60172-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    IF Staining of HeLa using 60172-1-Ig (same clone as 60172-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60172-1-PBS targets PCMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCMT1 fusion protein Ag6277 相同性解析による交差性が予測される生物種
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated molecular weight 25 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC008748
Gene symbol PCMT1
Gene ID (NCBI) 5110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.