Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-60172 targets PCMT1 in WB applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PCMT1 fusion protein Ag6277 相同性解析による交差性が予測される生物種 |
| Full Name | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25-27 kDa |
| GenBank accession number | BC008748 |
| Gene Symbol | PCMT1 |
| Gene ID (NCBI) | 5110 |
| RRID | AB_2920511 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP PCMT1 antibody HRP-60172 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |