PCNA Polyclonal antibody

Name: PCNA Polyclonal antibody
Brand: Proteintech
SKU: 24036-1-AP

PCNA Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24036-1-AP

Synonyms

proliferating cell nuclear antigen, Cyclin

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICC

WB analysis of HEK-293 using 24036-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 24036-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 24036-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24036-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 24036-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 24036-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 24036-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 24036-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 24036-1-AP
IP result of anti-PCNA (IP:24036-1-AP, 4ug; Detection:24036-1-AP 1:1000) with HEK-293 cells lysate 2000ug.
IHC staining of human gliomas using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).
IHC staining of human stomach cancer using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human stomach cancer using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).
IHC staining of human stomach cancer using 24036-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).
IF Staining of human breast cancer using 24036-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human breast cancer using 24036-1-AP
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 24036-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24036-1-AP (PCNA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 24036-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse testis tissue, Raji cells, HEK-293 cells, NIH/3T3 cells, MCF-7 cells, rat liver tissue, HepG2 cells, Jurkat cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human breast cancer tissue, human gliomas tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human breast cancer tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IHC	See 11 publications below
IF	See 7 publications below
RIP	See 1 publications below

Product Information

24036-1-AP targets PCNA in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PCNA fusion protein Ag7416 相同性解析による交差性が予測される生物種
Full Name	proliferating cell nuclear antigen
Calculated molecular weight	29 kDa/31 kDa
Observed molecular weight	36-38 kDa
GenBank accession number	BC000491
Gene symbol	PCNA
Gene ID (NCBI)	5111
RRID	AB_2879414
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.This antibody specifically react with the 36kd human PCNA protein.

Protocols

Product Specific Protocols
WB protocol for PCNA antibody 24036-1-AP	Download protocol
IHC protocol for PCNA antibody 24036-1-AP	Download protocol
IF protocol for PCNA antibody 24036-1-AP	Download protocol
IP protocol for PCNA antibody 24036-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Int J Biol Sci Tendon Cells Root Into (Instead of Attach to) Humeral Bone Head via Fibrocartilage-Enthesis Authors - Zheng Wang View Article
rat	WB,IF	Biochem Pharmacol Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo to Trigger Phenotypic Switching of Pulmonary Artery Smooth Muscle Cells Authors - Ning Huang View Article
rat	WB	FASEB J S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis. Authors - Chao Fang View Article
rat	WB,IHC	Biochem Pharmacol CGRP attenuates pulmonary vascular remodeling by inhibiting the cGAS-STING-NFκB pathway in pulmonary arterial hypertension Authors - Xin Yan View Article
human	WB	Am J Cancer Res YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis Authors - Ya-Ni Chen View Article
mouse	WB	Hepatol Commun Hepatocellular carcinoma cells downregulate NADH:Ubiquinone Oxidoreductase Subunit B3 to maintain reactive oxygen species homeostasis Authors - Zhendong Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCNA Polyclonal antibody

PCNA Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 24036-1-AP

WB analysis using 24036-1-AP

WB analysis of NIH/3T3 using 24036-1-AP

WB analysis of MCF-7 using 24036-1-AP

WB analysis of rat liver using 24036-1-AP

WB analysis of MCF-7 using 24036-1-AP

WB analysis of mouse testis using 24036-1-AP

WB analysis of Raji using 24036-1-AP

IP experiment of HEK-293 using 24036-1-AP

IHC staining of human gliomas using 24036-1-AP

IHC staining of human breast cancer using 24036-1-AP

IHC staining of human stomach cancer using 24036-1-AP

IHC staining of human gliomas using 24036-1-AP

IHC staining of human breast cancer using 24036-1-AP

IHC staining of human stomach cancer using 24036-1-AP

IHC staining of human stomach cancer using 24036-1-AP

IF Staining of human breast cancer using 24036-1-AP

IF Staining of human breast cancer using 24036-1-AP

IF Staining of HepG2 using 24036-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Tendon Cells Root Into (Instead of Attach to) Humeral Bone Head via Fibrocartilage-Enthesis

Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo to Trigger Phenotypic Switching of Pulmonary Artery Smooth Muscle Cells

S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.

CGRP attenuates pulmonary vascular remodeling by inhibiting the cGAS-STING-NFκB pathway in pulmonary arterial hypertension

YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis

Hepatocellular carcinoma cells downregulate NADH:Ubiquinone Oxidoreductase Subunit B3 to maintain reactive oxygen species homeostasis