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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCNA Recombinant antibody

PCNA Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240306B9

Cat no : 81302-6-RR

Synonyms

proliferating cell nuclear antigen, Cyclin, 240306B9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCNA Recombinant antibody (81302-6-RR)

    WB analysis using 81302-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81302-6-RR (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PCNA Recombinant antibody (81302-6-RR)

    IHC staining of human stomach cancer using 81302-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PCNA Recombinant antibody (81302-6-RR)

    IHC staining of human stomach cancer using 81302-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81302-6-RR (PCNA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PCNA Recombinant antibody (81302-6-RR)

    IF Staining of mouse testis using 81302-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PCNA antibody (81302-6-RR, Clone: 240306B9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using PCNA Recombinant antibody (81302-6-RR)

    FC experiment of HeLa using 81302-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCNA Recombinant antibody (81302-6-RR, Clone:240306B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, A431 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81302-6-RR is PCNA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCNA fusion protein Ag0277 相同性解析による交差性が予測される生物種
Full Name proliferating cell nuclear antigen
Calculated molecular weight 29 kDa/31 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000491
Gene symbol PCNA
Gene ID (NCBI) 5111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Protocols

Product Specific Protocols
WB protocol for PCNA antibody 81302-6-RRDownload protocol
IHC protocol for PCNA antibody 81302-6-RRDownload protocol
IF protocol for PCNA antibody 81302-6-RRDownload protocol
FC protocol for PCNA antibody 81302-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols