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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCOLCE Polyclonal antibody (28826-1-AP)

    WB analysis using 28826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28826-1-AP (PCOLCE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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PCOLCE Polyclonal antibody

PCOLCE Polyclonal Antibody for WB, ELISA
Cat No. 28826-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

PCOLCE, PCPE, PCPE 1, PCPE1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28826-1-AP targets PCOLCE in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCOLCE fusion protein Ag29719 相同性解析による交差性が予測される生物種
Full Name procollagen C-endopeptidase enhancer
Calculated molecular weight 48 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC000574
Gene Symbol PCOLCE
Gene ID (NCBI) 5118
RRIDAB_3086087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15113
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCOLCE (Procollagen C-endopeptidase enhancer 1), also named as PCPE1, is a secreted glycoprotein that functions as a positive regulator of procollagen processing. The calculated molecular weight of PCOLCE is 48 kDa. PCOLCE binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Procollagen C-proteinases have important roles in developmental processes and assembly of the extracellular matrix. It has been reported that the dysregulation of PCOLCE is involved in numerous diseases. For instance, the expression of PCOLCE positively correlates with muscle and liver fibrosis (PMID: 27458976). And PCOLCE was highly expressed in osteosarcoma and may play an important role in promoting the lung metastasis of osteosarcoma (PMID: 31285765).

Protocols

Product Specific Protocols
WB protocol for PCOLCE antibody 28826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols